PA5-117926

antibody from Invitrogen Antibodies

Targeting: EHD4 PAST4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117926

Provider

Invitrogen Antibodies

Product name

EHD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EHD4 Polyclonal Antibody (Product # PA5-117926) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: A431 Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate, Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EHD4 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 µL with EHD4 Polyclonal Antibody (Product # PA5-117926) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: EHD4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 72 kDa.