Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [2]
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Validation data
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- Product number
- RQ7717 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EHD4 Antibody / EH domain-containing protein 4
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the EHD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat pancreas, 2) rat heart, 3) rat testis, 4) rat C6, 5) mouse pancreas, 6) mouse heart, 7) mouse testis and 8) mouse RAW264.7 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey heart, 4) human placenta, 5) human Caco-2 and 6) human PC-3 cell lysate with EHD4 antibody. Predicted molecular weight ~61 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-251 cells with EHD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EHD4 antibody.