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- References [1]
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB100-41390 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-41390, RRID:AB_2095455
- Product name
- Goat Polyclonal DSCAM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_001380.2 and NP_996770.1).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak.
Li W, Guan KL
The Journal of biological chemistry 2004 Jul 30;279(31):32824-31
The Journal of biological chemistry 2004 Jul 30;279(31):32824-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dscam Antibody [NB100-41390] - (1ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DSCAM Antibody [NB100-41390] - Staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DSCAM Antibody [NB100-41390] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
