TA501243

antibody from OriGene

Targeting: PFN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501243

Provider

OriGene

Proper citation

OriGene Cat#TA501243, RRID:AB_11126016

Product name

Anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Antibody type

Monoclonal

Description

Anti-PFN1 (Profilin 1) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PFN1

Isotype

IgG

Antibody clone number

OTI2D2

Vial size

100 µl

Concentration

0.58 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFN1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (RC202338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PFN1 mouse monoclonal antibody (TA501243) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFN1(RC202338).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501243, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PFN1 antibody(TA501243),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PFN1 antibody(TA501243),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC