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LearnA300-149A
antibody from Invitrogen Antibodies
Targeting: MORC2
AC004542.C22.1, KIAA0852, ZCW3, ZCWCC1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- A300-149A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZCWCC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZCWCC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, HCT 116, and HT-29 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZCWCC1 antibody (Product # A300-149A lot 3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZCWCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCWCC1 antibody A300-149A (lot A300-149A-2) used for IP at 3 µg per reaction. ZCWCC1 was also immunoprecipitated by rabbit anti-ZCWCC1 antibody A300-150A. For blotting immunoprecipitated ZCWCC1, A300-149A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZCWCC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZCWCC1 antibody A300-149A (lot A300-149A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ZCWCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCWCC1 antibody (Product # A300-149A; Lot 3) used for IP at 6 µg per reaction. ZCWCC1 was also immunoprecipitated by a previous lot of this antibody (Product # A300-149A; Lot 2). For blotting immunoprecipitated ZCWCC1, Product # A300-149A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
