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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB600-236 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-236, RRID:AB_2145693
- Product name
- Rabbit Polyclonal MORC2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Involvement of histone deacetylation in MORC2-mediated down-regulation of carbonic anhydrase IX.
Shao Y, Li Y, Zhang J, Liu D, Liu F, Zhao Y, Shen T, Li F
Nucleic acids research 2010 May;38(9):2813-24
Nucleic acids research 2010 May;38(9):2813-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MORC2 Antibody [NB600-236] - Detection of human ZCWCC1 by Western blot and immunoprecipitation. Samples: HeLa nuclear extracts, Exposure time: 3 minutes (ECL)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MORC2 Antibody [NB600-236] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZCWCC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MORC2 antibody [NB600-236] used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MORC2 antibody NB600-236 used for IP at 3 ug per reaction. MORC2 was also immunoprecipitated by rabbit anti-MORC2 antibody NB600-237. For blotting immunoprecipitated MORC2, NB600-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
