GTX629448

antibody from GeneTex

Targeting: STAR STARD1

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629448

Provider

GeneTex

Product name

STAR antibody [GT868]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µl

References

Submitted references

JAK2/STAT3 signaling mediates IL-6-inhibited neurogenesis of neural stem cells through DNA demethylation/methylation.

Kong X, Gong Z, Zhang L, Sun X, Ou Z, Xu B, Huang J, Long D, He X, Lin X, Li Q, Xu L, Xuan A
Brain, behavior, and immunity 2019 Feb 11;

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Ultrastructural localization of 5-methylcytosine on DNA and RNA.

Masiello I, Biggiogera M
Cellular and molecular life sciences : CMLS 2017 Aug;74(16):3057-3064

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Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.

Shih CC, Liao MH, Hsiao TS, Hii HP, Shen CH, Chen SJ, Ka SM, Chang YL, Wu CC
PloS one 2016;11(9):e0163690

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Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats.

Hsieh MC, Lai CY, Ho YC, Wang HH, Cheng JK, Chau YP, Peng HY
Scientific reports 2016 Nov 18;6:37411

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Coordination of AUF1 and miR-148a destabilizes DNA methyltransferase 1 mRNA under hypoxia in endometriosis.

Hsiao KY, Wu MH, Chang N, Yang SH, Wu CW, Sun HS, Tsai SJ
Molecular human reproduction 2015 Dec;21(12):894-904

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dotblot analysis of5-Methylcytosine / 5-mC antibody [GT4111] with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-Methylcytosine / 5-mC antibody (GTX629448) at a dilution of 1:500. A. Unmethylated DNA B. DNA containing 5-methylcytosine C. DNA containing 5-hydroxymethylcytosine

Validation comment

Dot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Validation comment

Dot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX629448 5-Methylcytosine / 5-mC antibody [GT4111] Dot blot analysis of 5-Methylcytosine / 5-mC antibody [GT4111] with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-Methylcytosine / 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA B: DNA containing 5-methylcytosine C: DNA containing 5-hydroxymethylcytosine D: DNA containing 5-formylcytosine E: DNA containing 5-carboxylcytosine

Validation comment

Dot