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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00006770-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006770-M01, RRID:AB_2197667
- Product name
- Mouse Monoclonal StAR Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. STAR - steroidogenic acute regulator
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chemoprotective effects of kolaviron on ethylene glycol monoethyl ether-induced pituitary-thyroid axis toxicity in male rats.
Identification of StARD3 as a lutein-binding protein in the macula of the primate retina.
Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone.
Adedara IA, Farombi EO
Andrologia 2013 Apr;45(2):111-9
Andrologia 2013 Apr;45(2):111-9
Identification of StARD3 as a lutein-binding protein in the macula of the primate retina.
Li B, Vachali P, Frederick JM, Bernstein PS
Biochemistry 2011 Apr 5;50(13):2541-9
Biochemistry 2011 Apr 5;50(13):2541-9
Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone.
Sedelaar JP, Isaacs JT
The Prostate 2009 Dec 1;69(16):1724-9
The Prostate 2009 Dec 1;69(16):1724-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: StAR Antibody (5F9) [H00006770-M01] - Detection limit for recombinant GST tagged STAR is approximately 0.03ng/ml as a capture antibody.
