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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-33485 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33485, RRID:AB_2197666
- Product name
- Rabbit Polyclonal StAR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Heat stress during the luteal phase decreases luteal size but does not affect circulating progesterone in gilts1.
Bidne KL, Romoser MR, Ross JW, Baumgard LH, Keating AF
Journal of animal science 2019 Oct 3;97(10):4314-4322
Journal of animal science 2019 Oct 3;97(10):4314-4322
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: StAR Antibody [NBP1-33485] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: StAR Antibody [NBP1-33485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
