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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- MA5-47013 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STAR Monoclonal Antibody (HL1360)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution.
- Antibody clone number
- HL1360
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAR in Various tissue extracts (50 µg) separated by 15% SDS-PAGE. Membrane was blotted with STAR Monoclonal antibody (Product # MA5-47013) using a dilution of 1:3,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAR in Various whole cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with STAR Monoclonal antibody (Product # MA5-47013) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STAR in 293T whole cell extracts (30 µg) separated by 12% SDS-PAGE. Membrane was blotted with STAR Monoclonal antibody (Product # MA5-47013) using a dilution of 1:200,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of STAR in K562 cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR stained by StAR monoclonal antibody (Product # MA5-47013) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAR in paraffin-embedded mouse adrenal gland. Samples were incubated in STAR Monoclonal antibody (Product # MA5-47013). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAR in paraffin-embedded mouse kidney. Samples were incubated in STAR Monoclonal antibody (Product # MA5-47013). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAR in paraffin-embedded dog kidney. Samples were incubated in STAR Monoclonal antibody (Product # MA5-47013). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAR in paraffin-embedded cat kidney. Samples were incubated in STAR Monoclonal antibody (Product # MA5-47013). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
