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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-55654 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-POLE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NAVEKQPLSS NMIERSVVEA AVQECSQSVD ETIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES GSKFQLKTIE TLLGSTTKIG DAIVLG Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 90%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
Yenerall P, Das AK, Wang S, Kollipara RK, Li LS, Villalobos P, Flaming J, Lin YF, Huffman K, Timmons BC, Gilbreath C, Sonavane R, Kinch LN, Rodriguez-Canales J, Moran C, Behrens C, Hirasawa M, Takata T, Murakami R, Iwanaga K, Chen BPC, Grishin NV, Raj GV, Wistuba II, Minna JD, Kittler R
Cell chemical biology 2020 Jan 16;27(1):105-121.e14
Cell chemical biology 2020 Jan 16;27(1):105-121.e14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLE2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLE2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLE2 in human cell line A-431 shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a POLE2 Polyclonal Antibody (Product # PA5-55654).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of POLE2 in human cell line U-251 MG using a POLE2 Polyclonal Antibody (Product # PA5-55654) shows localization to nucleoplasm, nuclear bodies and vesicles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of POLE2 in human tonsil using a POLE2 Polyclonal Antibody (Product # PA5-55654) shows moderate nuclear positivity in germinal center and non-germinal center cells..
