PA5-112302

antibody from Invitrogen Antibodies

Targeting: BNIP1 Nip1, SEC20

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112302

Provider

Invitrogen Antibodies

Product name

BNIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BNIP1 using BNIP1 Polyclonal Antibody (Product # PA5-112302) at a dilution of 1:500. Lane A: HepG2 whole cell lysate, Lane B: Jurkat whole cell lysate, Lane C: U-251 MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BNIP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with BNIP1 Polyclonal Antibody (Product # PA5-112302) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BNIP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with BNIP1 Polyclonal Antibody (Product # PA5-112302) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of BNIP1 was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of BNIP1 Polyclonal Antibody (Product # PA5-112302) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.