Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-78576 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FSD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS Predicted Reactivity: Bovine (97%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.91 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FSD1 Polyclonal Antibody (Product # PA5-78576). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FSD1 Polyclonal Antibody (Product # PA5-78576) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FSD1 Polyclonal Antibody detects FSD1 protein at cytoskeleton by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FSD1 protein stained by FSD1 Polyclonal Antibody (Product # PA5-78576) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FSD1 Polyclonal Antibody detects FSD1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FSD1 Polyclonal Antibody (Product # PA5-78576) diluted at 1:1200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.