12515-1-AP

antibody from Invitrogen Antibodies

Targeting: NUFIP1 NUFIP, Rsa1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12515-1-AP

Provider

Invitrogen Antibodies

Product name

NUFIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MHAPGMKKI KLDTPEEIAR WREERRKNYP TLANIERKKK LKLEKEKRGA VLTTTQYGKM KGMSRHSQMA KIRSPGKNHK WKNDNSRQRA VTGSGSHLCD LKLEGPPEAN ADPLGVLINS DSESDKEEKP QHSVIPKEVT PALCSLMSSY GSLSGSESEP EETPIKTEAD VLAENQVLDS SAPKSPSQDV KATVRNFSEA KSENRKKSFE KTNPKRKKDY HNYQTLFEPR THHPYLLEML LAPDIRHERN VILQCVRYII KKDFFGLDTN SAKSKDV (1-276 aa encoded by BC017745)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.56 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 12515-1-AP (NUFIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 12515-1-AP (NUFIP1 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NUFIP1 (IP:12515-1-AP, 4ug; Detection:12515-1-AP 1:800) with HeLa cells lysate 1200ug.