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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-117337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GALNT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GALNT3 in Various whole cell extracts (30 µg). Samples were separated with 7.5% SDS-PAGE and incubated in GALNT3 polyclonal antibody (Product # PA5-117337) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GALNT3 in ice cold methyl hydroxide (5 min) fixed A431 cells (Green: GALNT3). Samples was incubated in GALNT3 polyclonal antibody (Product # PA5-117337) using a dilution of 1:500, followed by Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GALNT3 Polyclonal Antibody detects GALNT3 protein at Golgi by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GALNT3 stained by GALNT3 Polyclonal Antibody (Product # PA5-117337) diluted at 1:3,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GALNT3 Polyclonal Antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. GALNT3 stained by GALNT3 Polyclonal Antibody (Product # PA5-117337) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GALNT3 Polyclonal Antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. GALNT3 stained by GALNT3 Polyclonal Antibody (Product # PA5-117337) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GALNT3 Polyclonal Antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GALNT3 stained by GALNT3 Polyclonal Antibody (Product # PA5-117337) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GALNT3 Polyclonal Antibody detects GALNT3 protein at Golgi apparatus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GALNT3 stained by GALNT3 Polyclonal Antibody (Product # PA5-117337) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
