TA500357

antibody from OriGene

Targeting: MX1 IFI-78K, lncMX1-215, MxA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500357

Provider

OriGene

Proper citation

OriGene Cat#TA500357, RRID:AB_2147717

Product name

MX1 mouse monoclonal antibody, clone OTI2G12 (formerly 2G12)

Antibody type

Monoclonal

Description

MX1 mouse monoclonal antibody, clone OTI2G12 (formerly 2G12)

Host

Mouse

Conjugate

Unconjugated

Epitope

MX1

Isotype

IgG

Antibody clone number

OTI2G12

Vial size

100 µl

Concentration

0.3 mg/ml

References

Submitted references

The large GTPase Mx1 binds Kif5B for cargo transport along microtubules.

Ringer K, Riehl J, Müller M, Dewes J, Hoff F, Jacob R
Traffic (Copenhagen, Denmark) 2018 Dec;19(12):947-964

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MX1 (RC207418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 1 cell lines by using anti- MX1 monoclonal antibody at 1:200 dilution.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MX1 monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MX1 mouse monoclonal antibody (TA500357) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MX1(RC207418).

Validation comment

IF