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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB600-1399 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1399, RRID:AB_2207834
- Product name
- Rabbit Polyclonal TRAF7 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. Human (isoforms CRA_a and CRA_c).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antigen sequence
ELKFDVLDENQSKLSEDLMEFRR- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TRAF7 protein promotes Lys-29-linked polyubiquitination of IkappaB kinase (IKKgamma)/NF-kappaB essential modulator (NEMO) and p65/RelA protein and represses NF-kappaB activation.
Zotti T, Uva A, Ferravante A, Vessichelli M, Scudiero I, Ceccarelli M, Vito P, Stilo R
The Journal of biological chemistry 2011 Jul 1;286(26):22924-33
The Journal of biological chemistry 2011 Jul 1;286(26):22924-33
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: TRAF7 Antibody [NB600-1399] - Total protein from Huvec, MCF7, U2-OS, A431, SHSY-5Y and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAF7 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
