Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00009402-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009402-M01, RRID:AB_539331
- Product name
- Mouse Monoclonal GRAP2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GRAP2 - GRB2-related adaptor protein 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of GRAP2 expression in transfected 293T cell line by GRAP2 monoclonal antibody (M01), clone 1G12.Lane 1: GRAP2 transfected lysate(38 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRAP2 Antibody (1G12) [H00009402-M01] - GRAP2 monoclonal antibody (M01), clone 1G12 Analysis of GRAP2 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GRAP2 Antibody (1G12) [H00009402-M01] - Detection limit for recombinant GST tagged GRAP2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of GRAP2 transfected lysate using anti-GRAP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GRAP2 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRAP2 Antibody (1G12) [H00009402-M01] - Analysis of monoclonal antibody to GRAP2 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.