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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21963 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB3C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, mouse brain, rat brain.
- Concentration
- 0.94 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB3C was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAB3C Polyclonal Antibody (Product # PA5-21963) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAB3C Polyclonal Antibody (Product # PA5-21963). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RAB3C Polyclonal Antibody (Product # PA5-21963) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAB3C Polyclonal Antibody (Product # PA5-21963). Sample (30 µg of whole cell lysate). Lane A: H1299. 12% SDS PAGE. RAB3C Polyclonal Antibody (Product # PA5-21963) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RAB3C was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB3C Polyclonal Antibody (Product # PA5-21963) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using RAB3C (Product # PA5-21963) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
