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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2155 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2155, RRID:AB_1578992
- Product name
- PPM1F polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PPM1F.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of the multifunctional Ca2+/calmodulin-dependent protein kinase II by the PP2C phosphatase PPM1F in fibroblasts.
HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages.
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family.
Harvey BP, Banga SS, Ozer HL
The Journal of biological chemistry 2004 Jun 4;279(23):24889-98
The Journal of biological chemistry 2004 Jun 4;279(23):24889-98
HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages.
Izmailova E, Bertley FM, Huang Q, Makori N, Miller CJ, Young RA, Aldovini A
Nature medicine 2003 Feb;9(2):191-7
Nature medicine 2003 Feb;9(2):191-7
The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family.
Koh CG, Tan EJ, Manser E, Lim L
Current biology : CB 2002 Feb 19;12(4):317-21
Current biology : CB 2002 Feb 19;12(4):317-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PPM1F (arrow) using PPM1F polyclonal antibody (Cat # PAB2155). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PPM1F gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PPM1F polyclonal antibody (Cat # PAB2155) in mouse liver tissue lysate (35 ug/lane). PPM1F (arrow) was detected using the purified Polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PPM1F polyclonal antibody (Cat # PAB2155) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
