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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- R35386 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PDE4D Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids QPEACVIDDRSPDT were used as the immunogen for this PDE4D antibody. This sequence is common to PDE4D1 (~91kDa), PDE4D2 (~77kDa) and PDE4D3 (~85kDa).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the PDE4D antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of COS cell lysates with PDE4D antibody at 1ug/ml: 1) transfected with human PDE4D1 (~91kDa), 2) transfected with human PDE4D3 (~85kDa), 3) untransfected.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human heart tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skeletal muscle tissue with PDE4D antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
