Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-7183-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD218a (IL-18Ra) Monoclonal Antibody (H44), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The H44 antibody reacts with the interleukin-18 receptor alpha chain, also known as IL-1Rrp, an IL-1R family member. IL-18R alpha is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells. By itself, IL-18R alpha binds IL-18 with low affinity and when complexed with IL-18R beta chain binds IL-18 with high affinity. The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses. Applications Reported: This H44 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to stain whole blood before RBC lysis. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- H44
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references MAIT cells are activated during human viral infections.
Increased expression of interleukin-18 receptor on T lymphocytes in patients with acute graft-versus-host disease after allogeneic bone marrow transplantation.
Expression of interleukin (IL)-18 and functional IL-18 receptor on human vascular endothelial cells, smooth muscle cells, and macrophages: implications for atherogenesis.
A role for IL-18 in neutrophil activation.
An absolute requirement for STAT4 and a role for IFN-gamma as an amplifying factor in IL-12 induction of the functional IL-18 receptor complex.
Human IL-18 receptor and ST2L are stable and selective markers for the respective type 1 and type 2 circulating lymphocytes.
IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.
van Wilgenburg B, Scherwitzl I, Hutchinson EC, Leng T, Kurioka A, Kulicke C, de Lara C, Cole S, Vasanawathana S, Limpitikul W, Malasit P, Young D, Denney L, STOP-HCV consortium., Moore MD, Fabris P, Giordani MT, Oo YH, Laidlaw SM, Dustin LB, Ho LP, Thompson FM, Ramamurthy N, Mongkolsapaya J, Willberg CB, Screaton GR, Klenerman P
Nature communications 2016 Jun 23;7:11653
Nature communications 2016 Jun 23;7:11653
Increased expression of interleukin-18 receptor on T lymphocytes in patients with acute graft-versus-host disease after allogeneic bone marrow transplantation.
Fujimori Y, Yoshimoto T, Matsui K, Tsutsui H, Okamoto T, Kashiwamura S, Hada T, Okamura H, Kakishita E, Hara H, Nakanishi K
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2002 Jul;22(7):751-4
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2002 Jul;22(7):751-4
Expression of interleukin (IL)-18 and functional IL-18 receptor on human vascular endothelial cells, smooth muscle cells, and macrophages: implications for atherogenesis.
Gerdes N, Sukhova GK, Libby P, Reynolds RS, Young JL, Schönbeck U
The Journal of experimental medicine 2002 Jan 21;195(2):245-57
The Journal of experimental medicine 2002 Jan 21;195(2):245-57
A role for IL-18 in neutrophil activation.
Leung BP, Culshaw S, Gracie JA, Hunter D, Canetti CA, Campbell C, Cunha F, Liew FY, McInnes IB
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2879-86
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2879-86
An absolute requirement for STAT4 and a role for IFN-gamma as an amplifying factor in IL-12 induction of the functional IL-18 receptor complex.
Nakahira M, Tomura M, Iwasaki M, Ahn HJ, Bian Y, Hamaoka T, Ohta T, Kurimoto M, Fujiwara H
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1306-12
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1306-12
Human IL-18 receptor and ST2L are stable and selective markers for the respective type 1 and type 2 circulating lymphocytes.
Chan WL, Pejnovic N, Lee CA, Al-Ali NA
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1238-44
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1238-44
IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.
Tominaga K, Yoshimoto T, Torigoe K, Kurimoto M, Matsui K, Hada T, Okamura H, Nakanishi K
International immunology 2000 Feb;12(2):151-60
International immunology 2000 Feb;12(2):151-60
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD218a (IL-18 Receptor alpha) APC (right). Cells in the lymphocyte gate were used for analysis.