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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00001877-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001877-M03, RRID:AB_1214392
- Product name
- Mouse Monoclonal E4F1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. E4F1 - E4F transcription factor 1 (4B10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysatE (83.5 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: E4F1 Antibody (4B10) [H00001877-M03] - Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of monoclonal antibody to E4F1 on HeLa cell. Antibody concentration 10 ug/ml
