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LearnPA5-31241
antibody from Invitrogen Antibodies
Targeting: WBP11
BUG13, CFAP90, FAP90, NPWBP, PPP1R165, SIPP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-31241 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WBP11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji.
- Concentration
- 0.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WBP11 using 30 µg of A) Jurkat (B) Raji (C) K562 (D) THP-1 and E) HL-60 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WBP11 polyclonal antibody (Product # PA5-31241) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WBP11 using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WBP11 polyclonal antibody (Product # PA5-31241) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WBP11 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WBP11 Polyclonal Antibody (Product # PA5-31241) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of WBP11 was performed in paraffin-embedded mouse brain tissue using WBP11 Polyclonal Antibody (Product # PA5-31241) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Western BlotP11 (Product # PA5-31241) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotP11 Polyclonal Antibody detects Western BlotP11 protein at nucleus in rat adrenal by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal. Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotP11 Polyclonal Antibody detects Western BlotP11 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western BlotP11 Polyclonal Antibody immunoprecipitates Western BlotP11 protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of Western BlotP11 protein by 4 µg Western BlotP11 Polyclonal Antibody (Product # PA5-31241). 7.5 % SDS-PAGE. The immunoprecipitated Western BlotP11 protein was detected by Western BlotP11 Polyclonal Antibody (Product # PA5-31241) diluted at 1:1,000.
