PA1763

antibody from Boster Biological Technology

Targeting: ECI1 DCI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA1763

Provider

Boster Biological Technology

Product name

Anti-DCI/ECI1 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for DCI/ECI1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC, IF. Reactive species: Human. DCI/ECI1 information: Molecular Weight: 32816 MW; Subcellular Localization: Mitochondrion matrix.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Impacts of Dietary Pleurotus eryngii Polysaccharide on Nutrient Digestion, Metabolism, and Immune Response of the Small Intestine and Colon-An iTRAQ-Based Proteomic Analysis.

Ma G, Kimatu BM, Zhao L, Yang W, Pei F, Hu Q
Proteomics 2018 Apr;18(7):e1700443

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of DCI using anti-DCI antibody (PA1763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: A549 Whole Cell Lysate, Lane 4: SMMC Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: HELA Whole Cell Lysate, Lane 7: HT1080 Whole Cell Lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCI antigen affinity purified polyclonal antibody (Catalog # PA1763) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCI at approximately 33KD. The expected band size for DCI is at 33KD.

Additional image