HPA001808

antibody from Atlas Antibodies

Targeting: SAV1 salvador, WW45, WWP4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001808

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001808, RRID:AB_2666441

Product name

Anti-SAV1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

IGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGR
KYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFG
TYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQ
QTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYD
HILKWELFQLA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and A-549 using Anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line SiHa shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Sample type

HUMAN