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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB100-2580 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2580, RRID:AB_2092148
- Product name
- Rabbit Polyclonal DHX33 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DHX33 Antibody [NB100-2580] - Detection of Human DHX33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX33 antibody NB100-2580 used for IP at 3 mcg/mg lysate. DHX33 was also immunoprecipitated by rabbit anti-DHX33 antibody NB100-2581. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection by DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DHX33 Antibody [NB100-2580] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DHX33 Antibody [NB100-2580] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DHX33 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DHX33 Antibody [NB100-2580] - Human lung carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
