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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-93093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SQFAHYRRKQ MNLAYVKAAD CISEPVNREP PSREAQLLTL QNTSEFDILV IGGGATGSGC ALDAVTRGLK TALVERDDFS SGTSSRSTKL IHGGVRYLQK AIMKLDIEQY RMVKEALHER ANLLEIAPHL SAPLPIMLPV YKWWQLPYYW VGIKLYDLVA GSNCLKSSYV LSKSRALEHF PMLQKDKLVG AIVYYDGQHN DARMNLAIAL TAARYGAATA NYMEVVSLLK KTDPQTGKVR VSGARCKDVL TGQEFDVRAK CVINATGPFT DSVRKMDDKD AAAICQPSAG VHIVMPGYYS P; Positive Samples: MCF7, THP-1, SW480, Mouse skeletal muscle, Mouse liver, Mouse pancreas, Mouse brain; Cellular Location: Mitochondrion
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability.
Chakrabarti L, Chaerkady R, Wang J, Weng SHS, Wang C, Qian C, Cazares L, Hess S, Amaya P, Zhu J, Hatton D
mAbs 2022 Jan-Dec;14(1):2020081
mAbs 2022 Jan-Dec;14(1):2020081
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using GPD2 Polyclonal antibody (Product # PA5-93093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPD2 in extracts of various cell lines using GPD2 Polyclonal Antibody (Product # PA5-93093) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPD2 in paraffin-embedded mouse lung using GPD2 Polyclonal Antibody (Product # PA5-93093) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPD2 in paraffin-embedded human liver damage using GPD2 Polyclonal Antibody (Product # PA5-93093) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPD2 in paraffin-embedded human liver cancer using GPD2 Polyclonal Antibody (Product # PA5-93093) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPD2 in paraffin-embedded mouse lung using GPD2 Polyclonal Antibody (Product # PA5-93093) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 10. Western blot analysis of mitochondria and ER-associated proteins. (a) Whole cell lysates and mitochondrial lysates of parental CHO and MMP-enriched host cells were analyzed for proteins involved in mitochondrial function and high MMP phenotype. (b and c) Whole cell lysates of stable pools generated from parental CHO and MMP-enriched hosts were analyzed for mitochondrial and ER-stress proteins. Beta-actin and succinate dehydrogenase subunit A were used as loading controls. Western blot analysis of the lysates of host cells and pools demonstrating upregulation of proteins associated with mitochondrial functions and downregulation of ER-stress related proteins in MMP-enriched host cells and pools.
