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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [7]
- Other assay [1]
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Validation data
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- Product number
- 17219-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KAVKGTLVG GGALATVLGL SQFAHYRRKQ MNLAYVKAAD CISEPVNREP PSREAQLLTL QNTSEFDILV IGGGATGSGC ALDAVTRGLK TALVERDDFS SGTSSRSTKL IHGGVRYLQK AIMKLDIEQY RMVKEALHER ANLLEIAPHL SAPLPIMLPV YKWWQLPYYW VGIKLYDLVA GSNCLKSSYV LSKSRALEHF PMLQKDKLVG AIVYYDGQHN DARMNLAIAL TAARYGAATA NYMEVVSLLK KTDPQTGKVH VSGARCKDVL TGQEFDVRAK CVINATGPFT DSVRKMDDKD AAAICQPSAG VHIVMPGYYS PESMGLLDPA TSDGRVIFFL PWQKMTIAGT TDTPTDVTHH PIPSEEDINF ILNEV (5-378 aa encoded by BC019874)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17219-1-AP (GPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17219-1-AP (GPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17219-1-AP (GPD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 17219-1-AP (GPD2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 17219-1-AP) (GPD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GPD2 (IP:17219-1-AP, 3ug; Detection:17219-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug.
