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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA503937 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503937, RRID:AB_11128388
- Product name
- DPP9 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)
- Antibody type
- Monoclonal
- Description
- DPP9 mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DPP9
- Isotype
- IgG
- Antibody clone number
- OTI2F10
- Vial size
- 100 µl
- Concentration
- 0.67 mg/ml
Submitted references Dipeptidyl peptidase 9 enzymatic activity influences the expression of neonatal metabolic genes.
Dipeptidyl peptidase 9 subcellular localization and a role in cell adhesion involving focal adhesion kinase and paxillin.
Chen Y, Gall MG, Zhang H, Keane FM, McCaughan GW, Yu DM, Gorrell MD
Experimental cell research 2016 Mar 1;342(1):72-82
Experimental cell research 2016 Mar 1;342(1):72-82
Dipeptidyl peptidase 9 subcellular localization and a role in cell adhesion involving focal adhesion kinase and paxillin.
Zhang H, Chen Y, Wadham C, McCaughan GW, Keane FM, Gorrell MD
Biochimica et biophysica acta 2015 Feb;1853(2):470-80
Biochimica et biophysica acta 2015 Feb;1853(2):470-80
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (RC224465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of DPP9 protein expression by using anti-DPP9 antibody in Huh7 cells were transfected with DPP9 siRNA or nontargeting (NT) control siRNA for 48 h, or treated with enzyme inhibitor, 8 uM 1G244 (or vehicle control, 0.08% DMSO) for 6 h before incubating on fibronectin-coated plastic for 30 min. Dilution: 1:2000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-DPP9 mouse monoclonal antibody (TA503937) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP9(RC224465).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC224465 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DPP9 antibody(TA503937), and then analyzed by flow cytometry.
- Validation comment
- FC
