Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- CF503937 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DPP9 Monoclonal Antibody (OTI2F10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2F10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP9 (RC224465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9. Positive lysates LY403380 (100 µg) and LC403380 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of DPP9 protein expression by using anti-DPP9 antibody in Huh7 cells were transfected with DPP9 siRNA or nontargeting (NT) control siRNA for 48 h, or treated with enzyme inhibitor, 8 uM 1G244 (or vehicle control, 0.08% DMSO) for 6 h before incubating on fibronectin-coated plastic for 30 min. Dilution: 1:2000 View Citation
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-DPP9 mouse monoclonal antibody (TA503937) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP9(RC224465).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC224465 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP9 antibody (TA503937), and then analyzed by flow cytometry.
