AP7101A

antibody from Abgent

Targeting: ACVR1 ACVR1A, ACVRLK2, ALK2, SKR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7101A

Provider

Abgent

Proper citation

Abgent Cat#AP7101a, RRID:AB_2257850

Product name

Activin Receptor Type IA (ACVR1) Antibody (Center N153)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ACVR1 (arrow) using rabbit polyclonal ACVR1 Antibody (Center N153) (Cat.#AP7101a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVR1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ACVR1 Antibody (Cat. #AP7101a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ACVR1 antibody detected the ACVR1 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100