Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AF8555 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human alpha-1,4-N-Acetylglucosaminyltransferase in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9UNA3
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- 12 months from date of receipt, -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 °C under sterile conditions after opening.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO-III human gastric carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.