PA5-72939

antibody from Invitrogen Antibodies

Targeting: ABCG1 ABC8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

PA5-72939

Provider

Invitrogen Antibodies

Product name

ABCG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Hamster, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ABCG1 in mouse peritoneal macrophages. Samples were incubated in ABCG1 polyclonal antibody (Product # PA5-72939). Lane 1: untreated mouse peritoneal macrophages; Lane 2: 22-(R)-hydrocholesterol treated macrophages; Lane 3: T0901713 treated macrophages; Lane 4: T091713 treated macrophages.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ABCG1 in mouse peritoneal macrophages. Samples were incubated in ABCG1 polyclonal antibody (Product # PA5-72939). Lane 1: untreated mouse peritoneal macrophages; Lane 2: 22-(R)-hydrocholesterol treated macrophages; Lane 3: T0901713 treated macrophages; Lane 4: T091713 treated macrophages.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ABCG1 in Raw 264.7 (A), and Raw 264.7 + 10 µM T09 18 hr (B) cell lysate. Samples were incubated in ABCG1 polyclonal antibody (Product # PA5-72939 using a dilution of 2 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ABCG1 in human lung, alveolar macrophages. Samples were incubated in ABCG1 polyclonal antibody (Product # PA5-72939).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ABCG1 in RAW 246.7 cells were either untreated (A) or serum starved for 24 hours, and then treated with 1 µM TO9 for 24 hours (B). Samples were incubated in ABCG1 polyclonal antibody (Product # PA5-72939) using a dilution of 5 µg/mL for 30 minutes at room temperature followed by Goat Anti-Rabbit Dylight 550-conjugated antibody. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin.