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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-117821 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Carbonic Anhydrase XIV Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Carbonic Anhydrase XIV in Lane A: 293T Membrane Lysate Lysates/proteins at 20 μg per lane. Samples (20 µg per lane) were incubated with polyclonal antibody (Product # PA5-117821) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 38 kDa, Observed band size: 45 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Carbonic Anhydrase XIV Polyclonal Antibody (Product # PA5-117821) at 1:500 dilution. Lane A: 293T Membrane Lysate. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 45 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of Carbonic Anhydrase XIV in human liver. Samples were incubated with polyclonal antibody (Product # PA5-117821) with a dilution of 1:5,000 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human Carbonic Anhydrase XIV in human liver with Carbonic Anhydrase XIV Polyclonal Antibody (Product # PA5-117821, 1:5,000 dilution, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte. Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Carbonic Anhydrase XIV Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 0.5 µL with Carbonic Anhydrase XIV Polyclonal Antibody (Product # PA5-117821) and 15 µL of 50 % Protein G agarose. Primary antibody: Carbonic Anhydrase XIV Polyclonal Antibody, at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 42 kDa.
