Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C675501 - Provider product page
- Provider
- LSBio
- Product name
- CENPK Antibody LS-C675501
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot All Lanes:CENPK antibody at 3.98 ug/ml Lane 1: PC-3 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32,75 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating CENPK in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CENPK Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CENPK Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating CENPK in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CENPK Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CENPK Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating CENPK in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CENPK Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CENPK Antibody (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg)