PAB20044

antibody from Abnova Corporation

Targeting: DRG1 NEDD3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20044

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20044, RRID:AB_10961887

Product name

DRG1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DRG1.

Antigen sequence

ELEGFGIRLNSKPPNIGFKKKDKGGINLTATCPQS
ELDAETVKSILAEYKIHNADVTLRSDATADDLIDV
VEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCV
PISAHHRWNFDDLLEKIWDYLKLVRIYTKPKGQLP
DYT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: EFO-21, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with DRG1 polyclonal antibody (Cat # PAB20044).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with DRG1 polyclonal antibody (Cat # PAB20044).Lane 1 : NIH/3T3Lane 2 : NBT-IILane 3: PC-12

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with DRG1 polyclonal antibody (Cat # PAB20044) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with DRG1 polyclonal antibody (Cat # PAB20044) shows strong cytoplasmic positivity in purkinje cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)