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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502734 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502734, RRID:AB_11141167
- Product name
- ITM2B mouse monoclonal antibody, clone OTI1C11 (formerly 1C11)
- Antibody type
- Monoclonal
- Description
- ITM2B mouse monoclonal antibody, clone OTI1C11 (formerly 1C11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ITM2B
- Isotype
- IgG
- Antibody clone number
- OTI1C11
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (RC202377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ITM2B monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ITM2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502734)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ITM2B antibody(TA502734),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
