Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF7866 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Erbin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Erbin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse ErbB2 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Erbin by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Erbin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7866) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Erbin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.