PA5-30637

antibody from Invitrogen Antibodies

Targeting: UBASH3A CLIP4, STS-2, TULA

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30637

Provider

Invitrogen Antibodies

Product name

UBASH3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: THP-1.

Concentration

1 mg/mL

References

Submitted references

The T cell CD6 receptor operates a multitask signalosome with opposite functions in T cell activation.

Mori D, Grégoire C, Voisinne G, Celis-Gutierrez J, Aussel R, Girard L, Camus M, Marcellin M, Argenty J, Burlet-Schiltz O, Fiore F, Gonzalez de Peredo A, Malissen M, Roncagalli R, Malissen B
The Journal of experimental medicine 2021 Feb 1;218(2)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using UBASH3A Polyclonal Antibody (Product # PA5-30637). Sample (30 µg of whole cell lysate). Lane A: THP-1. 10% SDS PAGE. UBASH3A Polyclonal Antibody (Product # PA5-30637) diluted at 1:2,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Composition and dynamics of the CD6 signalosome of long-term expanded CD6 OST CD4 + T cells. (A) Volcano plot showing proteins significantly enriched after affinity purification in CD4 + T cells expressing CD6 OST molecules compared with affinity purification in control CD4 + T cells expressing similar levels of WT (untagged) CD6 proteins before (t 0s ) and at 30 s (t 30s ) and 120 s (t 120s ) after TCR plus CD4 stimulation. (B) Volcano plot showing proteins significantly enriched after affinity purification in CD6 OST -expressing CD4 + T cells 30 and 120 s after TCR engagement compared with affinity purification in unstimulated CD6 OST -expressing CD4 + T cells. For A and B, see description in Fig. 2 B . (C) Dot plot showing the interaction stoichiometry over the course of TCR stimulation of CD6 with its seven high-confidence preys, the interaction stoichiometry of which changed following TCR engagement. See description in Fig. 2 C . (D) Stoichiometry plot of the CD6 interactome. The CD6 bait is shown as a yellow dot. Red and blue dots correspond to preys that showed increased or decreased binding following TCR stimulation. The purple dot corresponds to a prey whose association was not regulated by TCR stimulation. See description in Fig. 2 D . (E) Biochemical validation of the CD6-UBASH3A and CD6-GRK6 interactions predicted on the basis of AP-MS analysis. Long-term-expanded WT (Control) and CD6 OST -expressing CD4 + T cells were left unstimulated (0) or were stimu