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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-56644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FRYL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AYCKLINQVN TIKNEAEVIN MSEELAQLES ILKEAESASE NEEIDISKAA QTTIETAIHS LIETLKNKEF ISAVAQVKA
- Concentration
- 0.2 mg/mL
Submitted references MicroRNA-1205 Regulation of FRYL in Prostate Cancer.
Naidoo M, Levine F, Gillot T, Orunmuyi AT, Olapade-Olaopa EO, Ali T, Krampis K, Pan C, Dorsaint P, Sboner A, Ogunwobi OO
Frontiers in cell and developmental biology 2021;9:647485
Frontiers in cell and developmental biology 2021;9:647485
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FRYL in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a FRYL Polyclonal Antibody (Product # PA5-56644).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FRYL in human cell line HEL using a FRYL Polyclonal Antibody (Product # PA5-56644).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FRYL in mouse cell line NIH-3T3 and rat cell line NBT-II using a FRYL Polyclonal Antibody (Product # PA5-56644).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FRYL in human cell line U-251 MG shows positivity in cytoplasm & microtubules. Samples were probed using a FRYL Polyclonal Antibody (Product # PA5-56644).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FRYL in human testis tissue shows strong cytoplasmic positivity in subsets of cells in seminiferus ducts. Samples were probed using a FRYL Polyclonal Antibody (Product # PA5-56644).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 FRYL is overexpressed in PCa and is regulated by miR-1205. (A) FRYL was significantly overexpressed in PCa tissues when compared to normal tissues {ANOVA [ F (1, 22) = 4.3, p = 0.009]. (B) Using thesame cohort of tissues in Figure 1A , FRYL overexpression was observed in PCa tissues when compared to normal prostatic tissues. (C) Western blot analysis of FRYL in CRPC C4-2B, small cell prostate carcinoma PC-3 cells and androgen-sensitive LNCaP cells. (D) FRYL protein levels in PC-3 cells after inhibition of miR-1205 with NB1205. Data is presented as +- SD.
