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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA1-41099 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XAF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: antigen standard for XAF1 (transient overexpression lysate), human liver tissue or protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease.
Zhu C, Li B, Frontzek K, Liu Y, Aguzzi A
The Journal of experimental medicine 2019 Apr 1;216(4):743-756
The Journal of experimental medicine 2019 Apr 1;216(4):743-756
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XAF1 in human liver lysate using a XAF-1 polyclonal antibody (Product # PA1-41099) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XAF1 in human liver lysate. Samples were incubated in XAF1 polyclonal antibody (Product # PA1-41099) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of XAF1 in immersion fixed paraffin-embedded sections of human appendix. Samples were incubated in XAF1 polyclonal antibody (Product # PA1-41099) using a dilution of 1:100 for 1 hour at room temperature followed by the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells.
