Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-112634 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OFD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.45 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OFD1 in mouse skeletal muscle tissue using a OFD1 polyclonal antibody (Product # PA5-112634) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OFD1 in mouse skeletal muscle tissue using a OFD1 polyclonal antibody (Product # PA5-112634) at a concentration of 2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OFD1 in multiple sample types using a OFD1 polyclonal antibody (Product # PA5-112634) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, SH-SY5Y whole cell lysate, and K562 whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of OFD1 in HepG2 cells using a Polyclonal antibody (Product # PA5-112634) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded OFD1 in human adrenal gland tissue using a OFD1 Polyclonal antibody (Product # PA5-112634) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of OFD1 in paraffin-embedded human skeletal muscle tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at 37°C, then the primary antibody (1% BSA) was incubated at 4°C overnight. The sample was incubated with a OFD1 polyclonal antibody (Product # PA5-112634) at a dilution of 1:100, followed by a goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.