PA5-30798

antibody from Invitrogen Antibodies

Targeting: SIL1 BAP, MSS, ULG5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30798

Provider

Invitrogen Antibodies

Product name

SIL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, rat kidney. Predicted reactivity: Chimpanzee (99%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.74 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SIL1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SIL1 Polyclonal Antibody (Product # PA5-30798) at a dilution of 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SIL1 Polyclonal Antibody (Product # PA5-30798). Sample (30 µg of whole cell lysate). Lane A: A431. 10% SDS PAGE. SIL1 Polyclonal Antibody (Product # PA5-30798) diluted at 1:5,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SIL1 was performed in paraffin-embedded rat muscle tissue using SIL1 Polyclonal Antibody (Product # PA5-30798) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using SIL1 (Product # PA5-30798) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.