PA5-117975

antibody from Invitrogen Antibodies

Targeting: GSTT2B GSTT2P

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117975

Provider

Invitrogen Antibodies

Product name

GSTT2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of GSTT2B in Lane A: Rat kidney tissue lysate, Lane B: Rat lung tissue lysate, Lane C: Rat pancreas tissue lysate, Lane D: Mouse lung tissue lysate,. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117975) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 27 kDa, Observed band size: 27 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GSTT2B Polyclonal Antibody (Product # PA5-117975) at 1:500 dilution. Lane A: Rat kidney tissue lysate, Lane B: Rat lung tissue lysate, Lane C: Rat pancreas tissue lysate, Lane D: Mouse lung tissue lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GSTT2B in Lane A: 0.5 mg Rat lung tissue Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117975) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 27 kDa , Observed band size : 25 kDa .