GTX115949

antibody from GeneTex

Targeting: TUBGCP4 76P, FLJ14797

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX115949

Provider

GeneTex

Proper citation

GeneTex Cat#GTX115949, RRID:AB_10621536

Product name

GCP4 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.

Cota RR, Teixidó-Travesa N, Ezquerra A, Eibes S, Lacasa C, Roig J, Lüders J
Journal of cell science 2017 Jan 15;130(2):406-419

---

The dynamics of microtubule minus ends in the human mitotic spindle.

Lecland N, Lüders J
Nature cell biology 2014 Aug;16(8):770-8

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GCP4 antibody detects TUBGCP4 protein by Western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract 10 % SDS-PAGEGCP4 antibody (GTX115949) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using GCP4(GTX115949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GCP4 antibody detects GCP4 protein at cytoplasm and centrosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GCP4 protein stained by GCP4 antibody (GTX115949) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.