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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-PW8830-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Proteasome 19S Rpt4/S10b subunit monoclonal antibody (p42-23)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Partially purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- p42-23
- Vial size
- 100 μl
- Storage
- -20°C
Submitted references SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes.
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Homma T, Kurahashi T, Lee J, Kang ES, Fujii J
Archives of biochemistry and biophysics 2015 Oct 1;583:65-72
Archives of biochemistry and biophysics 2015 Oct 1;583:65-72
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
Pitcher DS, de Mattos-Shipley K, Tzortzis K, Auner HW, Karadimitris A, Kleijnen MF
EBioMedicine 2015 Jul;2(7):642-8
EBioMedicine 2015 Jul;2(7):642-8
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Supportive validation
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- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: Composite luminograph of (a) HeLa S3 cytosolic preparation, (b) purified 26S proteasome, and (c) human placental proteasomefraction after SDS PAGE followed by blotting onto PVDF membrane and probing with antibody BML-PW8830. Antibody dilution 1:5000 using ECL procedure (1 min exposure).
