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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00081602-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00081602-M01, RRID:AB_537597
- Product name
- Mouse Monoclonal CDADC1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CDADC1 - cytidine and dCMP deaminase domain containing 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDADC1 Antibody (1A2) [H00081602-M01] - Analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi ( Cat # H00081602-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody (M01), clone 1A2 (Cat # H00081602-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDADC1 Antibody (1A2) [H00081602-M01] - Analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody (M01), clone 1A2.Lane 1: CDADC1 transfected lysate(58.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDADC1 Antibody (1A2) [H00081602-M01] - CDADC1 monoclonal antibody (M01), clone 1A2. Analysis of CDADC1 expression in Raw 264.7.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDADC1 Antibody (1A2) [H00081602-M01] - CDADC1 monoclonal antibody (M01), clone 1A2. Analysis of CDADC1 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CDADC1 Antibody (1A2) [H00081602-M01] - Detection limit for recombinant GST tagged CDADC1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CDADC1 Antibody (1A2) [H00081602-M01] - Analysis of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml.
