12392-1-AP

antibody from Invitrogen Antibodies

Targeting: A4GALT A14GALT, Gb3S, P(k), P1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12392-1-AP

Provider

Invitrogen Antibodies

Product name

A4GALT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HVVGEPKEK GQLYNLPAEI PCPTLTPPTP PSHGPTPGNI FFLETSDRTN PNFLFMCSVE SAARTHPESH VLVLMKGLPG GNASLPRHLG ISLLSCFPNV QMLPLDLREL FRDTPLADWY AAVQGRWEPY LLPVLSDASR IALMWKFGGI YLDTDFIVLK NLRNLTNVLG TQSRYVLNGA FLAFERRHEF MALCMRDFVD HYNGWIWGHQ GPQLLTRVFK KWCSIRSLAE SRACRGVTTL PPEAFYPIPW QDWKKYFEDI NPEELPRLLS ATYAVHVWNK KSQGTRFEAT SRALLAQLHA RYCPTTHEAM KMYL (41-353 aa encoded by BC017068)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP ( A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (Product # 12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 12392-1-AP (A4GALT antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).