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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-60313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CABIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EPGGKVGLLN HRPVAMDAGD SADQSGERKD KESPRAGPTE PMDTSEATVC HSDLERTPPL LPGRPARDRG PESRPTELSL E
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CABIN1 Polyclonal Antibody (Product # PA5-60313) and a 246 kDa band corresponding to Calcineurin-binding protein cabin-1 was observed across all the cell lines tested along with uncharacterized bands (*). Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), IMR-90 (Lane 2), HUVEC (Lane 3), HCT 116 (Lane 4), SH-SY5Y (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CABIN1 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a CABIN1 Polyclonal Antibody (Product # PA5-60313).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CABIN1 in human rectal tissue shows strong nuclear and cytoplasmic positivity in glandular cells. Samples were probed using a CABIN1 Polyclonal Antibody (Product # PA5-60313).
